Sample Prep
BI-209
The Proteome Exploration Laboratory has a dedicated sample preparation room that the staff and collaborators utilize to either stage samples for immediate LC-MS analysis or work through a series of established protocols to prepare the samples for analysis. We work with researchers both on and off campus, providing expert guidance and hands-on services for LC-MS analysis. BI-209 is equipped with a laminar flow hood, CentriVap lyophilizer system, and two Agilent 1100/1200 HPLC systems used for offline peptide/protein desalting and fractionation.
Vanquish UHPLC System
- stable flow rates over a wide range of chromatographic applications
- auto sampler, auto injector, column thermal regulation
- uv and fluorescence detectors
- automated fraction collection by time or peak
Complex sample fractionation | Sample cleanup
Mass Spectrometry
BI-206 and BI-208
The Proteome Exploration Laboratory has two specialized laboratories housing state-of-the-art mass spectrometers.
Orbitrap Fusion Tribrid Mass Spectrometer
- Ultra-high resolution for quantitation of isobaric compounds (TMT)
- Multiple fragmentation methods; CID, HCD, ETD and EThcD
- Targeted workflows to quantify very low level peptides
- Synchronous Precursor Selection
Orbitrap Eclipse Tribrid Mass Spectrometer
- Ultra-high resolution (500k)
- Top-down proteomics
- Multiple fragmentation methods: CID, HCD, ETD, EThcD and UVPD
- PTCR (Proton Transfer Charge Reduction)
- FAIMS (High-Field Asymmetric Waveform Ion Mobility Spectrometry )
- Real-Time Search and Synchronous Precursor Selection
Thermo Astral Mass Spectrometer
- Ultra-high resolution (500k)
- Ultra-fast scanning (up to 200Hz)
- Data Independent Acquisition (DIA)
- Constantly identifies over 9000 proteins with 50 SPD
- FAIMS (High-Field Asymmetric Waveform Ion Mobility Spectrometry)
Thermo Stellar Mass Spectrometer
- Ultra-fast scanning (up to 125Hz)
- Suitable for targeted proteomics
- Data Independent Acquisition (DIA) or Parallel Reaction Monitoring (PRM)
- Simultaneous and accurate quantitation for ~1000 peptides.